Quote (EndlessSky @ Aug 3 2022 05:46pm)
That is incorrect.
https://www.mdpi.com/1467-3045/44/3/73In the experiment itself, the Swedish scientists used separate laboratory samples of Integrase and Reverse transcriptase to insert the vaccine RNA into the DNA of laboratory liver cells.
The COVID vaccine cannot alter the genome on its own.
The papers garbage but they are claiming its an endogenous LINE-1 insertion. The reverse transcriptase you read about is just their qrtPCR assay to look at gene expression downstream.
Edit: Since I have time I can tell you why the papers garbage:
Their model is a liver cancer cell line that greatly over-expresses endogenous line-1 compared to normal liver cells which should be the first red flag that they're fishing for a sketchy result. The dosage of mRNA they're using is pretty extreme for a cell culture model, most things are 1000-fold less than what they use. For comparison, the pfizer vaccine is 30 ug of mRNA into the human body.
Figures 2/3 they use students T-test which is inappropriate for both the comparisons they're making. There are very few occasions in science where you can use students T and gene expression data isn't one of them.
Figure 4: This should have rejected the paper on its own, they've manipulated the images to hdie the line-1 expression at baseline which you can tell by the hoescht intensity which should be consistent across all 4 images.
Final point, looking at mRNA to look at whether or not your gene is integrated from mRNA is a really poor measure of integration. They never demonstrate their pfizer drug washed out from their cell cultures before showing increases in spike protein mRNA which is important since they added spike protein mRNA. It'd be pretty trivial to toss some primers in for your target and sanger sequence the genomic DNA up and downstream of your presumptive insertion to get an idea of where it went into the genome. The sequencing that they do inclue is just confirming the mRNA they use is the pfizer vaccine.
They're lacking proper controls which would be a scrambled mRNA sequence of similar length to the pfizer vaccine as opposed to empty vehicle.
This post was edited by Sioux on Aug 3 2022 07:16pm